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Photoshop - combine red and green channel images into a merged image?

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Hi,

 

I am a developmental biologist carrying out experiments on a type of microscope known as a Confocal Laser Scanning Microscope (CSLM).  Basically, this is a microscope coupled to some lasers which allow fluorescently-labelled parts of a biological sample to be imaged in high detail.

 

For each sample there will be a set of two images produced, named and numbered as follows:

 

sample01_g.tif

sample01_r.tif

 

Each image represents one fluorescent wavelength and correspond to green ( g ) and red ( r ).  These images are in rgb colour.

 

I want to produce a merge of these two channels to generate a new TIFF file such that:

 

Green channel is taken from sample01_g.tif

Red channel is taken from sample01_r.tif

 

Which would show me how the different channels co-localise, or not.

 

This is fine to do by hand in Photoshop, but takes a long time when you have a couple of hundred images to process.  So, what I would like to do is write a script that batch automates the process, like a droplet.  I have the process outlined below:

 

  1. Create a new RGB TIFF called sample01_merge.tif
  2. Take the green channel from sample01_g.tif and copy it to the green channel of sample01_merge.tif
  3. Take the red channel from sample01_r.tif and copy it to the red channel of sample01_merge.tif
  4. Save sample01_merge.tif into a folder named "Merged Images" on the desktop
  5. Close the opened images
  6. Go to next set of images –> sample02_g.tif and sample02_r.tif
  7. Create a new RGB TIFF called sample02_merge.tif....and go through step 2-6, untill the last set of images has been processed.

 

I have never used script before and would love to learn, but I am making slow progress and would like to have this problem solved sooner than I will be able to do so by myself.  So... I was wondering if anyone could give me some advice on how to go about doing this?

 

Any help will be greatly appreciated!

 

I use a Mac Pro running OSX 10.8.3 and Photoshop CS6 Extended

 

Best regards,

John


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